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Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Strategic Study of CAE 2000, Volume 2, Issue 11,   Pages 1-11

Abstract:

In this paper, the study on family collection, gene mapping, gene identification and gene functiont (1; 3) (q44; p11) associated with nasopharyngeal cancer was found in 1975 at first, and human TDF geneIn 1998, GJB3, a human genetic neurological deafness gene, was identified using a novel strategy of “Gene Family-Candidate Disease Gene Cloning”，and the paper was published in Nature Geneticswas also cloned from gene function study.

Keywords： genetic disease     gene mapping and cloning     gene familiy-candidate disease gene cloning     genome wide scan     gene function study    

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Recent advances in fruit crop genomics

Qiang XU,Chaoyang LIU,Manosh Kumar BISWAS,Zhiyong PAN,Xiuxin DENG

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 21-27 doi: 10.15302/J-FASE-2014002

Abstract: In recent years, dramatic progress has been made in the genomics of fruit crops. The publication of a dozen fruit crop genomes represents a milestone for both functional genomics and breeding programs in fruit crops. Rapid advances in high-throughput sequencing technology have revolutionized the manner and scale of genomics in fruit crops. Research on fruit crops is encompassing a wide range of biological questions which are unique and cannot be addressed in a model plant such as . This review summarizes recent achievements of research on the genome, transcriptome, proteome, miRNAs and epigenome of fruit crops.

Keywords： fruit crops     fruit biology     gene function     genomics    

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Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 663-675 doi: 10.1007/s11705-017-1623-5

Abstract: Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as long as they can be delivered safely and effectively into cells. Dendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecture and multivalent cooperativity. However, the clinical translation of RNA therapeutics mediated by dendrimer delivery is hampered by the lack of dendrimers that are of high quality to meet good manufacturing practice standard. In this context, we have developed small amphiphilic dendrimers that self-assemble into supramolecular structures, which mimic high-generation dendrimers synthesized with covalent construction, yet are easy to produce in large amount and superior quality. Indeed, the concept of supramolecular dendrimers has proved to be very promising, and has opened up a new avenue for dendrimer-mediated siRNA delivery. A series of self-assembling supramolecular dendrimers have consequently been established, some of them out-performing the currently available nonviral vectors in delivering siRNA to various cell types and , including human primary cells and stem cells. This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery and the advantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNA delivery. We then highlight our efforts in creating self-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA delivery as well as the related structural alterations to enhance delivery efficiency. The advent of self-assembling supramolecular dendrimer nanovectors holds great promise and heralds a new era of dendrimer-mediated delivery of RNA therapeutics in biomedical applications.

Keywords： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

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The antibiotic resistome: gene flow in environments, animals and human beings

Yongfei Hu, George F. Gao, Baoli Zhu

Frontiers of Medicine 2017, Volume 11, Issue 2,   Pages 161-168 doi: 10.1007/s11684-017-0531-x

Abstract: We propose that the resistance gene flow in nature is “from the natural environments” andand animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene

Keywords： antibiotic resistance     resistome     microbiome     gene flow    

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Reflections on the system of evaluation of gene-edited livestock

Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 211-217 doi: 10.15302/J-FASE-2019303

Abstract: functions regarding economic traits, and new materials for livestock breeding have been obtained through geneEvaluation is the most important component of the regulation of gene-edited livestock and is a crucialguarantee of public safety before the marketing of gene-edited animal products.Here, the system of evaluation of gene-edited livestock is discussed in terms of public safety and production

Keywords： evaluation     gene editing     livestock     performance     safety    

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Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 627-636 doi: 10.1007/s11684-020-0815-4

Abstract: Then, based on RNA-seq data from The Cancer Genome Atlas AML cohort, we developed a 300-gene signatureThis study revealed the distinct gene expression pattern of RUNX1 mutations and the underlying

Keywords： RUNX1     gene mutation     acute myeloid leukemia     transcriptional repression     DNA methylation    

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Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 141-147 doi: 10.1007/s11684-009-0041-6

Abstract: To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed LentivirusWestern blotting revealed that the Rab9 gene expression in BALB/c mice brain was up-regulated significantlyLentivirus encoding Rab9 can increase the expression of Rab9 gene effectively, which might offer a novel

Keywords： Rab9     lentivirus     gene therapy     gene transfer    

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Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation

Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5,   Pages 889-901 doi: 10.1007/s11705-020-1919-8

Abstract: Gene therapy has drawn great attention in the treatments of many diseases, especially for cardiovascularHowever, the development of gene carriers with low cytotoxicity and multitargeting function is stillThe high internalization efficiency of these gene complexes was confirmed by flow cytometry.The rapid migration of ECs transfected by these gene complexes was verified by wound healing assay.carrier with low cytotoxicity and high transfection efficiency has great potential in gene therapy.

Keywords： gene carriers     multitargeting function     ECs     transfection efficiency    

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Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 521-528 doi: 10.1007/s11705-017-1612-8

Abstract: The application of gene delivery materials has been mainly focused on mammalian cells while rarely extendedThis study therefore widens the utilities of helical polypeptide as a unique category of gene deliverymaterials, and may find their promising applications toward plant gene delivery.

Keywords： α-helical polypeptide     plant gene delivery     protoplast     intact leaves     transfection    

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Profiling influences of gene overexpression on heterologous resveratrol production in

Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 117-125 doi: 10.1007/s11705-016-1601-3

Abstract: We overexpressed the main gene targets in the upstream pathways to investigate their influences on theSingle-gene overexpression and DNA assembly-directed multigene overexpression affect the production ofFinally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.14The newly discovered gene targets expressing phosphoribosylanthranilate isomerase, expressing 3-deoxy-D-arabino-heptulosonate

Keywords： resveratrol     aromatic amino acid     DNA assembly     metabolic engineering     gene overexpression    

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Lung function and air pollution exposure in adults with asthma in Beijing: a 2-year longitudinal panel

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 574-583 doi: 10.1007/s11684-021-0882-1

Abstract: The effect of air pollution on the lung function of adults with asthma remains unclear to date.The participants were divided into three groups according to their lung function level at every visitA linear mixed-effect model was applied to predict the change in lung function with each unit changeExposure to CO and PM2.5 was negatively associated with the large airway function of adultsPatients with asthma react differently to air pollutants as evidenced by their lung function levels.

Keywords： lung function     asthma     air pollution     adult    

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Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity

Chuanfeng Wu, Cynthia E. Dunbar

Frontiers of Medicine 2011, Volume 5, Issue 4,   Pages 356-371 doi: 10.1007/s11684-011-0159-1

Abstract: Virus-based vectors are widely used in hematopoietic stem cell (HSC) gene therapy, and have the abilityTo date, HSC gene therapy has been successfully employed in the clinic for improving clinical outcomesHowever, adverse events were observed during some of these HSC gene therapy clinical trials, linked tomechanisms of insertional mutagenesis in hematopoietic stem and progenitor cells due to integrating gene, and introduce newly-developed approaches for minimizing genotoxicity as a way to further move HSC gene

Keywords： gene therapy     hematopoietic stem cells     insertional mutagenesis     genotoxicity     induced pluripotent stem cell    

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Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Frontiers of Medicine 2015, Volume 9, Issue 1,   Pages 90-99 doi: 10.1007/s11684-015-0390-2

Abstract:

Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B geneindex and adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-functionIn addition, the regulatory elements that control the FIX gene expression in these cassettes

Keywords： factor IX     hemophilia B     liver-specific regulatory elements     hydrodynamic gene transfer    

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Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome

Lu Wang

Frontiers of Medicine 2013, Volume 7, Issue 3,   Pages 280-289 doi: 10.1007/s11684-013-0265-3

Abstract:

Many gene fusions have been recognized as important diagnostic and/or prognostic markers in humanIn recent years, novel gene fusions have been identified in cases without prior knowledge of the geneticThis review focused on advanced genome-wide screening approaches in fusion gene identification, suchas microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression systemThe fundamental rationale and strategy for fusion gene identification using each biotech platform are

Keywords： gene fusion     cancer     microarray     next-generation sequencing     NanoString nCounter system    

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Potential functions of esophageal cancer-related gene-4 in the cardiovascular system

Rui Zhou, Yuanshu Liu, Wenjun Huang, Xitong Dang

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 639-645 doi: 10.1007/s11684-019-0701-0

Abstract: Esophageal cancer-related gene-4 ( ) is cloned from the normal epithelium of the esophagus.correlated with the malignant phenotype of tumor cells, validating that is a real tumor suppressor gene

Keywords： tumor suppressor gene     esophageal cancer-related gene-4     cardiovascular disease     hypoxia    

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Title Author Date Type Operation

Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Journal Article

Recent advances in fruit crop genomics

Qiang XU,Chaoyang LIU,Manosh Kumar BISWAS,Zhiyong PAN,Xiuxin DENG

Journal Article

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Journal Article

The antibiotic resistome: gene flow in environments, animals and human beings

Yongfei Hu, George F. Gao, Baoli Zhu

Journal Article

Reflections on the system of evaluation of gene-edited livestock

Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI

Journal Article

Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Journal Article

Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Journal Article

Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation

Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng

Journal Article

Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Journal Article

Profiling influences of gene overexpression on heterologous resveratrol production in

Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan

Journal Article

Lung function and air pollution exposure in adults with asthma in Beijing: a 2-year longitudinal panel

Journal Article

Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity

Chuanfeng Wu, Cynthia E. Dunbar

Journal Article

Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Journal Article

Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome

Lu Wang

Journal Article

Potential functions of esophageal cancer-related gene-4 in the cardiovascular system

Rui Zhou, Yuanshu Liu, Wenjun Huang, Xitong Dang

Journal Article